Journal: Journal of Hematology & Oncology

Article Title: Challenges and advances in clinical applications of mesenchymal stromal cells

doi: 10.1186/s13045-021-01037-x

Figure Lengend Snippet: The tests of modified MSCs using CRISPR-Cas9 technology

Article Snippet: Immortalized human bone marrow MSC cell line (ATCC PCS-500–041) , PUMILIO2 (PUM2) , Depletion of PUM2 blocks MSC adipogenesis and enhances osteogenesis. PUM2 works as a negative regulator on the 3′ UTRs of JAK2 and RUNX2 via direct binding. CRISPR/CAS9-mediated gene silencing of Pum2 inhibited lipid accumulation and excessive bone formation , [ ] .

Techniques: Modification, CRISPR, Functional Assay, Synthesized, In Vitro, Transfection, Expressing, Transplantation Assay, Inhibition, Binding Assay

Journal: bioRxiv

Article Title: Surface potential as a strong early osteogenic trigger via mechanotransduction and calcium accumulation

doi: 10.64898/2026.04.26.720950

Figure Lengend Snippet: Colorimetric staining and quantification of ALP activity in hBM-MSCs cultured for seven days on collagen type I-coated (a) and fibronectin-coated (b) substrates. Results are expressed as mean ± SD (n=3). Data were analyzed assuming a Gaussian (normal) distribution and equal variances across groups; statistical differences were assessed using one-way ANOVA. Only p-values <0.1 are shown. Scale bar represents 200 µm.

Article Snippet: hBM-MSCs (Cytion, 300665) were seeded at an approximate density of 5.000 cells/cm 2 on the different surfaces in basal medium (DMEM; 11995-065, Gibco), 10% fetal bovine serum (FBS; 11560636, Gibco), 1% Glutamax (13462629, Gibco) and 1% antibiotics (11548876, Gibco), and after 24 hours the culture medium was replaced with osteogenic medium (Basal medium supplemented with 0,28 mM ascorbic acid (A4544, Sigma-Aldrich), 10 mM β-glycerol-2-phosphate (G9422, Sigma-Aldrich), and 10 nM dexamethasone (D4902, Sigma-Aldrich)).

Techniques: Staining, Activity Assay, Cell Culture

Journal: bioRxiv

Article Title: Surface potential as a strong early osteogenic trigger via mechanotransduction and calcium accumulation

doi: 10.64898/2026.04.26.720950

Figure Lengend Snippet: Fluorescence images of hBM-MSCs cultured on β-PVDF films coated with either collagen type I (a) or fibronectin (b) and corresponding morphological features. Nuclei are stained in blue, vinculin in green and F-actin in red. Results are expressed as mean ± SD (n=3). Only p-values <0.1 are shown. Scale bars represent 200 µm.

Article Snippet: hBM-MSCs (Cytion, 300665) were seeded at an approximate density of 5.000 cells/cm 2 on the different surfaces in basal medium (DMEM; 11995-065, Gibco), 10% fetal bovine serum (FBS; 11560636, Gibco), 1% Glutamax (13462629, Gibco) and 1% antibiotics (11548876, Gibco), and after 24 hours the culture medium was replaced with osteogenic medium (Basal medium supplemented with 0,28 mM ascorbic acid (A4544, Sigma-Aldrich), 10 mM β-glycerol-2-phosphate (G9422, Sigma-Aldrich), and 10 nM dexamethasone (D4902, Sigma-Aldrich)).

Techniques: Fluorescence, Cell Culture, Staining

Journal: bioRxiv

Article Title: Surface potential as a strong early osteogenic trigger via mechanotransduction and calcium accumulation

doi: 10.64898/2026.04.26.720950

Figure Lengend Snippet: MYPT1 phosphorylation in hBM-MSCs cultured on collagen type I-coated (a) and fibronectin-coated (b) β-PVDF films of varying surface potential. Results are expressed as mean ± SD (n=3). Only p-values <0.1 are shown.

Article Snippet: hBM-MSCs (Cytion, 300665) were seeded at an approximate density of 5.000 cells/cm 2 on the different surfaces in basal medium (DMEM; 11995-065, Gibco), 10% fetal bovine serum (FBS; 11560636, Gibco), 1% Glutamax (13462629, Gibco) and 1% antibiotics (11548876, Gibco), and after 24 hours the culture medium was replaced with osteogenic medium (Basal medium supplemented with 0,28 mM ascorbic acid (A4544, Sigma-Aldrich), 10 mM β-glycerol-2-phosphate (G9422, Sigma-Aldrich), and 10 nM dexamethasone (D4902, Sigma-Aldrich)).

Techniques: Phospho-proteomics, Cell Culture

Journal: bioRxiv

Article Title: Surface potential as a strong early osteogenic trigger via mechanotransduction and calcium accumulation

doi: 10.64898/2026.04.26.720950

Figure Lengend Snippet: Fluorescence images of hBM-MSCs cultured on β-PVDF films coated with either collagen type I (a) or fibronectin (b) immunostained against YAP and counterstained with Hoechst 33342, and the corresponding quantifications (right panels). Scale bars represent 200 µm. Results are expressed as mean ± SD (n=3). Only p-values <0.1 are shown.

Article Snippet: hBM-MSCs (Cytion, 300665) were seeded at an approximate density of 5.000 cells/cm 2 on the different surfaces in basal medium (DMEM; 11995-065, Gibco), 10% fetal bovine serum (FBS; 11560636, Gibco), 1% Glutamax (13462629, Gibco) and 1% antibiotics (11548876, Gibco), and after 24 hours the culture medium was replaced with osteogenic medium (Basal medium supplemented with 0,28 mM ascorbic acid (A4544, Sigma-Aldrich), 10 mM β-glycerol-2-phosphate (G9422, Sigma-Aldrich), and 10 nM dexamethasone (D4902, Sigma-Aldrich)).

Techniques: Fluorescence, Cell Culture

Journal: bioRxiv

Article Title: Surface potential as a strong early osteogenic trigger via mechanotransduction and calcium accumulation

doi: 10.64898/2026.04.26.720950

Figure Lengend Snippet: Volcano plots with the −log 10 (p-value) plotted against their respective log 2 (fold change) of genes differentially expressed in hBM-MSCs, Venn diagrams and histogram plots showing genes that are down- or upregulated (p<0.05) in hBM-MSCs cultured on the indicated surfaces for 24 hours (a) or four days (b). Circle area in a and b is proportional to the number of genes. GSEA of the cells cultured on the different surfaces for 24 hours (c) and 4 days (d).

Article Snippet: hBM-MSCs (Cytion, 300665) were seeded at an approximate density of 5.000 cells/cm 2 on the different surfaces in basal medium (DMEM; 11995-065, Gibco), 10% fetal bovine serum (FBS; 11560636, Gibco), 1% Glutamax (13462629, Gibco) and 1% antibiotics (11548876, Gibco), and after 24 hours the culture medium was replaced with osteogenic medium (Basal medium supplemented with 0,28 mM ascorbic acid (A4544, Sigma-Aldrich), 10 mM β-glycerol-2-phosphate (G9422, Sigma-Aldrich), and 10 nM dexamethasone (D4902, Sigma-Aldrich)).

Techniques: Cell Culture

Journal: bioRxiv

Article Title: Surface potential as a strong early osteogenic trigger via mechanotransduction and calcium accumulation

doi: 10.64898/2026.04.26.720950

Figure Lengend Snippet: (a) Immunostaining of hBM-MSCs cultured on the different β-PVDF surfaces with glutaraldehyde-crosslinked collagen type I coating and corresponding morphologic analysis (b). MYTP1 phosphorylation (c) and YAP translocation (d and e) in hBM-MSCs cultured on the substrates. Only p-values <0.1 are shown. Scale bars represent 200 µm. Results are expressed as mean ± SD (n=3).

Article Snippet: hBM-MSCs (Cytion, 300665) were seeded at an approximate density of 5.000 cells/cm 2 on the different surfaces in basal medium (DMEM; 11995-065, Gibco), 10% fetal bovine serum (FBS; 11560636, Gibco), 1% Glutamax (13462629, Gibco) and 1% antibiotics (11548876, Gibco), and after 24 hours the culture medium was replaced with osteogenic medium (Basal medium supplemented with 0,28 mM ascorbic acid (A4544, Sigma-Aldrich), 10 mM β-glycerol-2-phosphate (G9422, Sigma-Aldrich), and 10 nM dexamethasone (D4902, Sigma-Aldrich)).

Techniques: Immunostaining, Cell Culture, Phospho-proteomics, Translocation Assay

Journal: bioRxiv

Article Title: Surface potential as a strong early osteogenic trigger via mechanotransduction and calcium accumulation

doi: 10.64898/2026.04.26.720950

Figure Lengend Snippet: Immunofluorescent images (a) and corresponding morphologic analysis (b) of hBM-MSCs treated with the ROCK inhibitor Y-27632 for 24 hours. YAP immunolocalization (c) and translocation (d) in treated cells. Only p-values <0.1 are shown. Scale bars represent 300 µm. Results are expressed as mean ± SD (n=4).

Article Snippet: hBM-MSCs (Cytion, 300665) were seeded at an approximate density of 5.000 cells/cm 2 on the different surfaces in basal medium (DMEM; 11995-065, Gibco), 10% fetal bovine serum (FBS; 11560636, Gibco), 1% Glutamax (13462629, Gibco) and 1% antibiotics (11548876, Gibco), and after 24 hours the culture medium was replaced with osteogenic medium (Basal medium supplemented with 0,28 mM ascorbic acid (A4544, Sigma-Aldrich), 10 mM β-glycerol-2-phosphate (G9422, Sigma-Aldrich), and 10 nM dexamethasone (D4902, Sigma-Aldrich)).

Techniques: Translocation Assay

Journal: Heliyon

Article Title: MSCs-laden silk Fibroin/GelMA hydrogels with incorporation of platelet-rich plasma for chondrogenic construct

doi: 10.1016/j.heliyon.2023.e14349

Figure Lengend Snippet: A schematic illustration of the preparation of MSCs-laden gelatin methacrylate/silk fibroin (GelMA/SF, SG) hydrogel with the incorporation of PRP for treating KOA to reconstruct cartilage.

Article Snippet: Due to the potential differentiation into cartilage cells (chondrocytes), bone marrow-derived mesenchymal stem cells (MSCs, Normal, Human, ATCC®PCS-500-012TM) were used in this work.

Techniques:

Journal: Heliyon

Article Title: MSCs-laden silk Fibroin/GelMA hydrogels with incorporation of platelet-rich plasma for chondrogenic construct

doi: 10.1016/j.heliyon.2023.e14349

Figure Lengend Snippet: The MSCs proliferation after loaded in SG hydrogels and cultured for 72 h, cells cultured without hydrogel as a control group. Laser scanning confocal microscope (LSCM) images of MSCs loaded in SG-1 ( A , B , and C ), SG-2 (D, E, and F), and SG-3 ( G , H , and I ) hydrogels, and without hydrogels ( J , K , and L ) cultured for 72 h, images ( A , D , G , and J ) displayed that cell nucleus stained using DAPI as shown in blue color, images ( B , E , H , and K ) displayed that cytoskeleton stained using Phalloidin-FITC as shown in green color, and image ( C , F , I , and L ) was merged from DAPI, and Phalloidin-FITC stained images, respectively. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Due to the potential differentiation into cartilage cells (chondrocytes), bone marrow-derived mesenchymal stem cells (MSCs, Normal, Human, ATCC®PCS-500-012TM) were used in this work.

Techniques: Cell Culture, Control, Microscopy, Staining

Journal: Heliyon

Article Title: MSCs-laden silk Fibroin/GelMA hydrogels with incorporation of platelet-rich plasma for chondrogenic construct

doi: 10.1016/j.heliyon.2023.e14349

Figure Lengend Snippet: Cell viability of MSCs after loaded in SG hydrogels and cultured for different days. * p < 0.05, and ** p < 0.01.

Article Snippet: Due to the potential differentiation into cartilage cells (chondrocytes), bone marrow-derived mesenchymal stem cells (MSCs, Normal, Human, ATCC®PCS-500-012TM) were used in this work.

Techniques: Cell Culture

Journal: Heliyon

Article Title: MSCs-laden silk Fibroin/GelMA hydrogels with incorporation of platelet-rich plasma for chondrogenic construct

doi: 10.1016/j.heliyon.2023.e14349

Figure Lengend Snippet: Optical micrographs of ( A-H ) H&E and ( I–P ) Masson staining slices of tissues after SG hydrogels ( C, D, K, and L ), SG hydrogels incorporated with PRP ( E, F, M, and N ), and MSCs-laden SG hydrogels incorporated with PRP ( G, H, O, and P ) were intra-articular injected into SD rats for 8 weeks, respectively, the SD rats without hydrogels intervention as the control group ( A, B, I, and J ).

Article Snippet: Due to the potential differentiation into cartilage cells (chondrocytes), bone marrow-derived mesenchymal stem cells (MSCs, Normal, Human, ATCC®PCS-500-012TM) were used in this work.

Techniques: Staining, Injection, Control

Journal: Heliyon

Article Title: MSCs-laden silk Fibroin/GelMA hydrogels with incorporation of platelet-rich plasma for chondrogenic construct

doi: 10.1016/j.heliyon.2023.e14349

Figure Lengend Snippet: The obtained scores for SG hydrogel, SG hydrogel with PRP, MSCs-loaded hydrogel with PRP, and control group using Mankin Scoring system. * p < 0.05, and ** p < 0.01.

Article Snippet: Due to the potential differentiation into cartilage cells (chondrocytes), bone marrow-derived mesenchymal stem cells (MSCs, Normal, Human, ATCC®PCS-500-012TM) were used in this work.

Techniques: Control

Journal: Molecular medicine reports

Article Title: Long non‑coding RNA SNHG14 affects the proliferation and apoptosis of childhood acute myeloid leukaemia cells by modulating the miR‑193b‑3p/MCL1 axis.

doi: 10.3892/mmr.2020.11729

Figure Lengend Snippet: Figure 1. SNHG14 gene expression is upregulated in bone marrow tissues of patients with AML and AML cell lines. (A) Relative expression of SNHG14 in 57 AML bone marrow tissues and NBM. (B) Relative expression of SNHG14 in AML cell lines and human normal bone marrow CD34+ cells. **P<0.01 vs. CD34+ cells. The 2‑ΔΔCq method was used to analyse the relative mRNA expression level using GAPDH as an internal control. AML, acute myeloid leukaemia; NBM, normal marrow tissues; SNHG14, small nucleolar RNA host gene 14.

Article Snippet: Human normal bone marrow CD34+ cells and AML cell lines (MV‐4‐11, AML‐193, HL‐60, and KG‐1 cells) were obtained from the American Type Culture Collection and cultured in Dulbecco's modified Eagle's medium (DMEM; HyClone; GE Healthcare) containing 10% FBS (Gibco; Thermo Fisher Scientific, Inc.) at 37°C.

Techniques: Gene Expression, Expressing, Control

Journal: Molecular medicine reports

Article Title: Long non‑coding RNA SNHG14 affects the proliferation and apoptosis of childhood acute myeloid leukaemia cells by modulating the miR‑193b‑3p/MCL1 axis.

doi: 10.3892/mmr.2020.11729

Figure Lengend Snippet: Figure 3. miR‑193b‑3p is a target of SNHG14 in AML cells. (A) Starbase was used to predict the binding site between SNHG14 and miR‑193b‑3p. (B) Relative expression of miR‑193b‑3p in MV‑4‑11 and AML‑193 cells following SNHG14 silencing. **P<0.01 vs. blank control. (C) The target association between SNHG14 and miR‑193b‑3p was determined using an RNA immunoprecipitation assay. **P<0.01 vs. Anti‑IgG. (D) The target association between SNHG14 and miR‑193b‑3p was determined using a dual luciferase reporter gene assay. **P<0.01 vs. miR‑NC. (E) Relative expression of miR‑193b‑3p in 57 AML bone marrow tissues and NBM was detected by RT‑qPCR. (F) Spearman's correlation analysis was performed to evaluate the correlation between SNHG14 and miR‑193b‑3p expression. (G) Relative expression of miR‑193b‑3p in AML cell lines and human normal bone marrow CD34+ cells. **P<0.01 vs. CD34+ cells. The 2‑ΔΔCq method was used to analyse the relative mRNA expression level using U6 as an internal control. SNHG14, small nucleolar RNA host gene 14; miR, microRNA; si, small interfering RNA; NC, negative control; wt, wildtype; mut, mutated; NBM, normal marrow tissues; AGO2, protein argonaute‑2; AML, acute myeloid leukaemia.

Article Snippet: Human normal bone marrow CD34+ cells and AML cell lines (MV‐4‐11, AML‐193, HL‐60, and KG‐1 cells) were obtained from the American Type Culture Collection and cultured in Dulbecco's modified Eagle's medium (DMEM; HyClone; GE Healthcare) containing 10% FBS (Gibco; Thermo Fisher Scientific, Inc.) at 37°C.

Techniques: Binding Assay, Expressing, Control, RNA Immunoprecipitation, Luciferase, Reporter Gene Assay, Small Interfering RNA, Negative Control

Journal: Molecular medicine reports

Article Title: Long non‑coding RNA SNHG14 affects the proliferation and apoptosis of childhood acute myeloid leukaemia cells by modulating the miR‑193b‑3p/MCL1 axis.

doi: 10.3892/mmr.2020.11729

Figure Lengend Snippet: Figure 5. miR‑193b‑3p targets MCL1 in AML cells. (A) TargetScan was used to predict the binding site between miR‑193b‑3p and MCL1. (B) A dual luciferase reporter gene assay was employed to verify the target association between miR‑193b‑3p and MCL1. **P<0.01 vs. miR‑NC. (C) Relative expression of MCL1 in 57 AML bone marrow tissues and NBM was detected by RT‑qPCR. (D) Spearman's correlation analysis was performed to evaluate the correlation between MCL1 and miR‑193b‑3p expression. (E) Spearman's correlation analysis was performed to evaluate the correlation between MCL1 and SNHG14 expression. (F) Relative expression of miR‑193b‑3p in AML cell lines and human normal bone marrow CD34+ cells. **P<0.01 vs. CD34+ cells. The 2‑ΔΔCq method was used to analyse the relative mRNA expression level using GAPDH as an internal control. SNHG14, small nucleolar RNA host gene 14; miR, microRNA; NC, negative control; wt, wildtype; mut, mutated; NBM, normal marrow tissues; AML, acute myeloid leukaemia; MCL1, MCL1 apoptosis regulator BCL2 family member.

Article Snippet: Human normal bone marrow CD34+ cells and AML cell lines (MV‐4‐11, AML‐193, HL‐60, and KG‐1 cells) were obtained from the American Type Culture Collection and cultured in Dulbecco's modified Eagle's medium (DMEM; HyClone; GE Healthcare) containing 10% FBS (Gibco; Thermo Fisher Scientific, Inc.) at 37°C.

Techniques: Binding Assay, Luciferase, Reporter Gene Assay, Expressing, Control, Negative Control

Journal: Current Research in Pharmacology and Drug Discovery

Article Title: Role of pro-inflammatory cytokines in Alzheimer's disease and neuroprotective effects of pegylated self-assembled nanoscaffolds

doi: 10.1016/j.crphar.2022.100149

Figure Lengend Snippet: Effect of Memp, Marketed, (MEM-PLGA) SANs, (PEG-MEM-PLGA) SANs and (PEG-MEM-PLGA)-BMSc SANs on pro-inflammatory cytokines (a) IL-1β (b) IL-6 (c) IL-10 and (d) TNF-α observed in serum, cerebral, hepatic and renal tissues of treatment groups of scopolamine induced AD model vs. control and disease control (untreated cells) group and Data are represented as Mean ​± ​SEM, (n ​= ​3).

Article Snippet: HighQCTM Human Bone Marrow Derived Stem Cell (BMSc) obtained from ACCEGEN BIOTECHNOLOGY were grown in Minimum Essential Media (MEM).

Techniques: Control